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Apparent kinetic parameters for GOase variants with HMF.
| Variant Name | kcat,app (s−1) | KM,app (mM) | kcat/KM (s−1 M−1) |
|---|---|---|---|
| M3–5 | 123.9 ± 1.4 | 3.3 ± 0.1 | 38000 ± 1000 |
| M4 | 132.7 ± 2.6 | 2.1 ± 0.2 | 63000 ± 6000 |
| M5-1 | 153.9 ± 4.2 | 2.0 ± 0.2 | 77000 ± 8000 |
| M5-2 | 139.0 ± 2.4 | 1.8 ± 0.1 | 77000 ± 4000 |
| M6-A | 200.5 ± 13.7 | 10.6 ± 2.8 | 19000 ± 5000 |
| M6-B | 115.4 ± 6.6 | 15.8 ± 2.9 | 7300 ± 1400 |
| M7-1A | 246.2 ± 10.7 | 15.3 ± 2.2 | 16000 ± 2000 |
| M7-2A | 204.6 ± 10.7 | 14.5 ± 2.6 | 14000 ± 3000 |
