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. 2021 Aug 16;12:4946. doi: 10.1038/s41467-021-25034-3

Table 3.

Kinetic parameters of GOase variants determined in the TiTR with HMF.

Variant KMO (mM) KMS (mM) kcat (s−1) kcat/KMO (M−1 s−1) kcat/KMS (M−1 s−1)
M1 0.16 ± 0.11 53 ± 18 23.5 ± 4.7 (1.5 ± 1.1) × 105 (0.04 ± 0.02) × 104
M3–5a 1.39 ± 0.46 14.9 ± 4.5 651 ± 132 (4.7 ± 1.8) × 105 (4.4 ± 1.6) × 104
M4 0.37 ± 0.07 1.83 ± 0.30 120 ± 6 (3.3 ± 0.6) × 105 (6.6 ± 1.1) × 104
M6-A 0.15 ± 0.02 6.19 ± 0.41 166 ± 4 (10.9 ± 1.5) × 105 (2.7 ± 0.2) × 104

KMO: KM for oxygen, KMS: KM for HMF. aMeasured using CFE powder with an estimated GOase content, giving a higher uncertainty in the kcat parameter. Data previously published in the PhD thesis of A. T. P.77.