Table 5.

Conversion of HMF to DFF by GOase variant M₄ CFE at high HMF loading in the presence of different cosolvents.

Entry	GOase Variant	GOase (g/L)	GOase Form	HRP (g/L)	Co-Solvent	[HMF] (g/L)	Type HMF	Conversion (%)	Over-oxidation (%)
1	M4	0.625	CFE	0.0128	EtOAc	50	Crude	82	0.8
2	M4	0.625	CFE	0.0128	DEC	50	Crude	82	0.8
3	M4	0.625	CFE	0.0128	BuOAc	50	Crude	76	0.7

Data for reactions with purified enzyme provided in Supplementary Table 11. Conditions listed in the table highlight the main differences between samples, while those listed below the table apply to all entries unless otherwise stated.

Conditions: 0.05 mM CuSO₄ in GOase M₄ CFE reactions, 880 U/mL Catalase, 100 mM NaPi, 200 µL (80% of aqueous volume) solvent overlay, 20 °C, 6 h.