. 2021 Aug 16;12:4946. doi: 10.1038/s41467-021-25034-3

Table 6.

Conversion of 100 g/L semi-crude HMF to DFF by GOase variants.

Entry	GOase Variant	Conversion at 6 h (%)	Conversion at 24 h (%)
1/2	M₄	31	31
3/4	M_5-1	38	39
5/6	M_5-2	51	54
7/8	M_6-A	57	62
9/10	M_6-B	59	76
11/12	M_7-1A	64	69
13/14	M_7-2A	77	77
15/16	M_7-1B	64	81
17/18	M_7-2B	58	78

Conditions: 0.05 g/L pure enzyme, 0.0064 g/L HRP, 880 U/mL Catalase, 100 mM NaPi, 100 g/L Crude HMF in DEC (BASF), 200 µL (80% of aqueous volume) DEC, 20 °C, 6 and 24 h. Over-oxidation in all samples was found to be <0.3%.