Table 2.
Effects of treatment (noninjected; diluent-injected; injected with 2.4 μg of vitamin D3 (D3) or 25-hydroxycholecalciferol (25OHD3); and 2.4 μL of D3 and 25OHD3) on egg weight; percentage egg weight loss (PEWL) from 0 to 12 d of incubation (doi); hatchability of injected live embryonated (HI) eggs; hatchling BW; late, pip, and post-pip embryo mortalities; and hatchling mortality at 21 doi.
| In ovo injection treatment | N | Egg weight (g) | PEWL (%) | HI (%) | Hatchling BW (g) | Late embryo mortality1 (%) | Pip embryo mortality 2 (%) | Post-pip embryo mortality 3 (%) | Hatchling mortality 4 (%) |
|---|---|---|---|---|---|---|---|---|---|
| Noninjected5 | 10 | 60.8 | 6.7 | 96.3 | 42.9 | 1.1 | 1.7 | 0 | 0.6 |
| Diluent6 | 10 | 60.9 | 6.4 | 92.3 | 43.2 | 3.7 | 1.3 | 0 | 2.5 |
| D37 | 10 | 61.1 | 6.3 | 97.3 | 43.5 | 2.3 | 0 | 0 | 1.1 |
| 25OHD38 | 10 | 61.4 | 6.4 | 94.9 | 43.2 | 2.9 | 1.8 | 0.6 | 0 |
| D3+25OHD39 | 10 | 61.8 | 7.0 | 95.5 | 43.0 | 2.5 | 1.9 | 0 | 0 |
| Pooled SEM | 0.30 | 0.26 | 1.75 | 0.27 | 1.93 | 0.73 | 0.29 | 1.65 | |
| P-value | 0.171 | 0.243 | 0.304 | 0.427 | 0.747 | 0.340 | 0.445 | 0.555 |
Mortality between 18 doi (432 h of incubation) and 21 doi (502 h of incubation) prior to pip.
Mortality during the pipping process at 21 doi.
Mortality after the pipping process at 21 doi.
Mortality immediately after complete emergence of hatchlings from the shell at 21 doi.
Eggs that were not injected with diluent.
Eggs injected with 50 μL of commercial diluent at 18 doi.
Eggs injected with 50 μL of commercial diluent containing 2.4 μg of vitamin D3 at 18 doi.
Eggs injected with 50 μL of commercial diluent containing 2.4 μg of 25OHD3 at 18 doi.
Eggs injected with 50 μL of commercial diluent containing 2.4 μg of D3 and 2.4 μg of 25OHD3 at 18 doi.