Table 3.
Effects of treatment (noninjected; diluent-injected; injected with 2.4 μg of vitamin D3 (D3) or 25-hydroxycholecalciferol (25OHD3); and 2.4 μL of D3 and 25OHD3) BW, BW gain (BWG), average daily gain (ADG), feed intake (FI), average daily feed intake (ADFI), and total mortality through 41 d of age (doa).
| N | BW (g) | BWG1 (g) | ADG (g) | FI (g) | ADFI (g) | FCR (g/g) | ||
|---|---|---|---|---|---|---|---|---|
| In ovo injection treatment | Starter (0–14 doa) | |||||||
| Noninjected1 | 8 | 427 | 385 | 27.5 | 496 | 35.4 | 1.29 | |
| Diluent2 | 8 | 410 | 368 | 26.3 | 485 | 34.6 | 1.34 | |
| D33 | 8 | 405 | 363 | 25.9 | 488 | 34.8 | 1.35 | |
| 25OHD34 | 8 | 421 | 378 | 27.0 | 497 | 35.5 | 1.32 | |
| D3+25OHD35 | 8 | 419 | 376 | 26.9 | 513 | 36.6 | 1.37 | |
| Pooled SEM | 9.5 | 14.2 | 1.01 | 15.2 | 1.08 | 0.042 | ||
| P-value | 0.508 | 0.489 | 0.490 | 0.332 | 0.330 | 0.288 | ||
| BW (g) | BWG (g) | ADG (g) | FI (g) | ADFI (g) | FCR (g/g) | |||
| Grower (15–28 doa) | ||||||||
| Diluent | 8 | 1,390 | 980 | 70.0 | 1,623 | 116 | 1.67 | |
| D3 | 8 | 1,398 | 993 | 70.9 | 1,746 | 125 | 1.79 | |
| 25OHD3 | 8 | 1,469 | 1048 | 74.8 | 1,702 | 122 | 1.63 | |
| D3+25OHD3 | 8 | 1,434 | 1014 | 72.5 | 1,750 | 125 | 1.74 | |
| Pooled SEM | 31.5 | 28.1 | 2.00 | 67.2 | 4.8 | 0.081 | ||
| P-value | 0.282 | 0.353 | 0.353 | 0.513 | 0.512 | 0.518 | ||
| BW (g) | BWG (g) | ADG (g) | FI (g) | ADFI (g) | FCR (g/g) | |||
| Finisher (29–41 doa) | ||||||||
| Diluent | 8 | 2,925 | 1,523b | 117b | 2,870 | 122 | 1.93 | |
| D3 | 8 | 2,943 | 1,544b | 119b | 2,954 | 127 | 1.91 | |
| 25OHD3 | 8 | 3,096 | 1,665a | 128a | 3,032 | 128 | 1.83 | |
| D3+25OHD3 | 8 | 3,054 | 1,620ab | 125ab | 3,041 | 129 | 1.88 | |
| Pooled SEM | 80.4 | 51.2 | 3.9 | 115.2 | 3.7 | 0.091 | ||
| P-value | 0.098 | 0.030 | 0.030 | 0.578 | 0.578 | 0.730 | ||
| BW (g) | BWG (g) | ADG (g) | FI (g) | ADFI (g) | FCR (g/g) | |||
| 0–41 doa | ||||||||
| Diluent | 8 | 2,925 | 2,881 | 70.3 | 4977 | 226 | 1.70 | |
| D3 | 8 | 2,943 | 2,900 | 70.7 | 5188 | 227 | 1.76 | |
| 25OHD3 | 8 | 3,096 | 3,053 | 74.5 | 5232 | 233 | 1.68 | |
| D3+25OHD3 | 8 | 3,054 | 3,011 | 73.4 | 5304 | 234 | 1.74 | |
| Pooled SEM | 80.4 | 83.0 | 1.43 | 145.5 | 5.0 | 0.063 | ||
| P-value | 0.098 | 0.100 | 0.100 | 0.431 | 0.578 | 0.741 | ||
Treatment means within the same column within effect with no common superscripts are significantly different (P < 0.05).
Eggs that were not injected with diluent and that were also not challenged with coccidiosis at 14 doa.
Eggs injected with 50 μL of commercial diluent at d 18 of incubation (doi) and the subsequent coccidiosis challenge of chicks at 14 doa.
Eggs injected with 50 μL of commercial diluent containing 2.4 μg of vitamin D3 at 18 doi and the subsequent coccidiosis challenge of chicks at 14 doa.
Eggs injected with 50 μL of commercial diluent containing 2.4 μg of 25OHD3 at 18 doi and the subsequent coccidiosis challenge of chicks at 14 doa.
Eggs injected with of 50 μL of commercial diluent containing 2.4 μg of D3 and 2.4 μg of 25OHD3 at 18 doi and the subsequent coccidiosis challenge of chicks at 14 doa.