Figure 3.
Cellular uptake of the Aβ peptides studied by flow cytometry. (A) Sequence of the mirror-image peptide pair (MIPP) Aβ peptides tested. (B) Mean FACS results in SH-SY5Y cells normalized against L-Aβ40 (5 μM peptide, 15 h incubation). Bars show mean fluorescence with error bars for SD of three biological replicates. (C) Mean FACS results in HEK293T cells with and without PrPC expression, normalized against L-Aβ40 (5 μM peptide, 2 h incubation). Bars show mean fluorescence with error bars for SD of two biological replicates. Image reproduced with permission from Proc. Natl. Acad. Sci. U S A 2020, 117 (46), 28625–28631