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. 2021 Aug 16;11:16580. doi: 10.1038/s41598-021-96217-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of major Ca²⁺ handling proteins in human iPSC-derived cardiomyocytes by western blot analysis. (A) Western blot analysis of human cardiomyocytes showed that these cells express all major proteins involved in Ca²⁺ transport and regulation of the cardiac calcium pump SERCA2a. We analyzed the expression of the following proteins: RyR, ryanodine receptor; SERCA2a, the cardiac isoform of sarcoplasmic reticulum Ca²⁺-ATPase; Ca_v1.2, voltage-dependent L-type Ca²⁺ channel, α-1C subunit; NCX1, Na⁺–Ca²⁺ exchanger; PLN, phospholamban. (B) Detection of phosphorylated phospholamban (PLN) at positions Ser16 (PLN-pSer16) and Thr17 (PLN-pThr17) in response to pharmacological β2-adrenergic stimulation with isoproterenol (ISO, 1 µM); untreated cells were included in the analysis (basal, negative control). In all cases, we used a pig heart membrane preparation as a control (‘CSR’ lane) for the expression of Ca²⁺ handling proteins tested in this study.