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. 2021 Aug 16;4:974. doi: 10.1038/s42003-021-02498-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Immunoblot analysis of transiently expressed mitochondria-targeted Mt-DsRed2 and cytosol-targeted AcGFP in LONP1-depleted cells. HeLa cells were retransfected 3 d after the first siRNA transfection. Transfection of the pDsRed2-Mito and pAcGFP vectors was performed 2 d after the second siRNA transfection. Cells were collected every 3 h following plasmid transfection. Total, soluble (S) and insoluble (I) proteins from cells were prepared as described in the Methods and in Fig. 1b. a Immunoblot analysis of total proteins from the cells. b Immunoblot analysis of soluble (S) and insoluble (I) proteins from the cells. c The protein solubility of Mt-DsRed2 was quantified as the percentage of soluble proteins in the sum of soluble and insoluble proteins (n = 3).