Figure 2.

Validation of sgRNAs against PKD1 and PKD2. (A) Schematic presentation of the target sites from previous report²³ and our study is shown. (B) Outline of the experimental procedure used for validation of the sgRNAs. Cells were seeded in 96-well format and Cas9 expression was induced before transfection with sgRNA-expressing plasmids. (C) The resulting Cas9-induced mutations at the target sites were measured using deep targeted sequencing. Mutations were further analyzed for their effect on the reading frame.