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. 2021 Aug 3;8:704030. doi: 10.3389/fnut.2021.704030

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Copyright © 2021 Sun, Yue, Liu, Ma and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Correlation analysis and iron staining. (A) Linear regression and coefficients of determination (R²) between the total abundances of Bacillus spp. and content of three nutrients; (B) Perls' Prussian blue staining of grains. All data were normalized to their respective counterparts in the control group (NC) without inoculation of bacteria. In spraying, soils were sprayed with B. altitudinis WR10 before sowing of wheat; in soaking, wheat seeds were soaked in B. altitudinis WR10 suspension before sowing.