Figure 5.

CBX4 occupancy and H2AK119ub modification of the GnRH promoter in GT1-7 cells treated with maleate

(A) Venn diagram view of genes with significantly different enrichments of CBX4 and H2AK119ub. Red and green fonts indicate up- and downregulation of CBX4 and H2AK119ub enrichment in maleate versus control. Gene browser views of (B) H2AK119ub and (C) CBX4 enrichment on AGTPBP1, CCNG2, FOXK1, GNRH1, MEF2C, and RBP4 in maleate-treated GT1-7 cells. (D) KEGG pathway analysis of intersected genes in Venn diagram. (E) Enrichments of CBX4, H2AK119ub, and POLR2A in the GnRH promoter from ARC of PNW-3, -4, and -8 by ChIP-qPCR. The qPCR data are presented as the mean ± SEM of three separate experiments. ∗ represents the significant difference with a p value less than 0.05 compared to PNW-3 by one-way ANOVA.