Figure 3.

miR-132 inhibition prevents CCl₄-induced increase in EVs in the plasma

Mice received the treatments as described in Figure 2. Total number of extracellular vesicles (EVs) was measured from plasma using NanoSight as described in the Materials and methods (n = 5). (A) Size distribution of vesicles isolated from plasma after CCl₄ treatment by NTA analysis. (B) Extracellular vesicles were isolated using ExoQuick solution as described in the Materials and methods and used for electron microscopy. Representative picture is shown. (C) NTA analysis of extracellular vesicles after miR-132 inhibition. (D) Caspase 3 activity was determined from liver cell lysate using colorimetric assay as described in the Materials and methods . Fold change was calculated using saline-oil-treated mice. (E) Expression of Foxo3 and Bim was quantified by real-time PCR, and 18S was used to normalize Ct values. Data are shown as mean ± SEM. Mann-Whitney test or one-way ANOVA was employed for statistical analysis. ∗p < 0.05 compared to mice treated with oil and saline. #p < 0.05 compared to LNA-scrambled control-treated mice after CCl₄ treatment.