Figure 5.

miR-132 increase in Kupffer cells (KCs) and hepatocytes after CCl₄ treatment

Mice received the treatments as described in PFigure 2. (A–D) Total RNA was extracted from KCs and hepatocytes, and expression of miR-132 (A and C) and miR-212 (B and D) was quantified using TaqMan microRNA assay. SnoRNA-202 was used as internal control. (E) MMP-12 expression was measured from KCs using quantitative real-time PCR, and 18S was used to normalize Ct values. Fold change was calculated using cells isolated from saline-oil-treated mice. (F–I) RAW macrophages were transfected with either control or miRNA-132 mimic or inhibitor, as described in the Materials and methods . For the last 24 h of transfection, cells were either treated or not with 0.1% CCl₄, and expression of miR-132 (F), SIRT1 (G), IL-1β mRNA (H), and protein (I) was analyzed by quantitative real-time PCR and ELISA. Data are shown as mean ± SEM (n = 3). Mann-Whitney test or one-way ANOVA was employed for statistical analysis. ∗p < 0.05 compared to cells isolated from saline-oil-treated mice (A–E). ∗∗p < 0.005, ∗∗∗p < 0.0005, ∗∗∗∗p < 0.0001 (F–I). ns, non-significant.