Fig. 7.

HY1702 extenuates the lipopolysaccharide-induced activation of nuclear factor κB and mitogen-activated protein kinase in RAW264.7 macrophages. RAW264.7 cell were pretreated with HY1702 for 1 h and further treated with lipopolysaccharide for 24 h, at 6 h, protein was extracted for experiments. A: Expression of phosphorylated-IKBα/β, phosphorylated-IkBα/β in cytoplasm and nuclear phosphorylated-p65 level. B: The phosphorylated and total extracellular signal–regulated kinase (ERK) and p38 levels were determined by Western blot analysis. Total ERK (ERK), total p38 (p38), or total p65 (p65) protein was used as the loading control. C: The nuclear translocation of nuclear factor κB p65 was visualized by immunofluorescence. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; (blue)). Scale bar = 200 μm. The values presented are mean ± S.E.M of three independent experiments. ^###P < 0.001, ^##P < 0.01 compared with control; *P < 0.05, **P < 0.01, ***P < 0.001 compared with LPS mode. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)