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. 2021 Aug 6;2021:3225439. doi: 10.1155/2021/3225439

Figure 2.

Figure 2

Inflammatory cytokines promoted the activation of tRAS in human nucleus pulposus cells in vitro. (a, b) Secretion amount of Ang II in supernatant of human NP cells induced by IL-1β or TNF α was quantified by the ELISA assay (n = 5). (c, d) Western blot revealed the relative protein expression of tRAS components (ACE and AT1) in human NP cells induced by IL-1β or TNF α (n = 3). (e, f) RT-qPCR quantified the relative mRNA expression tRAS components (ACE and AT1) in human NP cells induced by IL-1β or TNF α in a time-change manner (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.