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. 2021 Aug 3;8:704598. doi: 10.3389/fvets.2021.704598

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Copyright © 2021 Rutland, Cockcroft, Lothion-Roy, Harris, Jeyapalan, Simpson, Alibhai, Bailey, Ballard-Reisch, Rizvanov, Dunning, de Brot and Mongan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Osteosarcoma GLUT1 immunohistochemical staining. (A–C) GLUT1 indicating positive nuclear and cytoplasmic immunostaining, immunopositive blood vessels with more pronounced cytoplasmic staining in the tunica intima in comparison to the tunica media, and negative staining in the osteoid and in (D) a tumor-free area. (E) Negative control OSA tissue showing no positive immunostaining. (F) Positive control canine kidney tissue staining primarily in the tubular epithelial cells. Scale bars represent 100 μm.