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Osteosarcoma immunohistochemical staining for NRF2. (A, B) Immunopositive staining in blood vessels, primarily nuclear, in all 10 samples within the tunica intima and in 40% of samples tunica media staining was observed. (C) Negative staining in the osteoid (tumor free area). [(D)+inset] Muscle tissue showing positive NRF2 staining. Scale bars represent (A) 100 μm, (B–D) 50 μm.
