Figures 11-14.

Mucopolysaccharidosis IIIB, dog, 26 months old. Figure 11. Cerebral cortex (postcruciate gyrus). (a) Moderate to severe cytoplasmic vacuolation of perivascular glia. Cytoplasmic vacuoles are large and clear. Hematoxylin and eosin (HE). (b) The vacuoles contain small periodic acid-Schiff (PAS)-positive inclusions (arrows). (c) Perivascular glial cells are compatible with microglia based on diffuse cytoplasmic immunolabelling for Iba1. Figure 12. Cerebellar cortex. (a) Severe cytoplasmic vacuolation of Purkinje cells. Cytoplasmic vacuoles are up to 1-μm-diameter, clear, and variably contain eosinophilic inclusions. HE. (b,c) The vacuoles contain PAS-positive (b) and Luxol fast blue-positive (LFB) (c) inclusions. Figure 13. Lateral vestibular nucleus. (a) There are cytoplasmic vacuoles that are small, punctate to granular, and located eccentrically. HE. (b-c) Neurons contain inclusions that stain positively with PAS (b) and LFB (c). Figure 14. Dorsal root ganglion. (a) Cytoplasmic vacuolation (arrowheads) of a neuron. Satellite glial cells (*) contain large, clear cytoplasmic vacuoles. HE. (b,c) The vacuoles contain PAS-positive (b) and LFB-positive (c) inclusions.