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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Hypertens Res. 2021 Apr 5;44(6):628–641. doi: 10.1038/s41440-021-00646-w

Fig. 6.

Fig. 6

Autophagy protein expression and mito-ROS production in mouse kidney cortices. A Autophagy marker protein expression in kidney cortices of Drd5−/− mice and their wild-type (Drd5+/+) littermates. The immunoblots show decreased protein expression of the autophagy marker proteins ATG5 and LC3-II in the kidney cortices of Drd5−/− mice relative to Drd5+/+ littermates. n = 4/group, †P < 0.05 vs Drd5+/+. B H2O2 production in isolated mitochondria from kidney cortices of Drd5−/− mice and their Drd5+/+ littermates. Veh vehicle, Fen fenoldopam (1.0 μM, 12 h), Sch Sch23390 (1.0 μM, 12 h), AA antimycin A (0.75 μM, 2 h), Cat catalase (10 μM, 30 min prior to AA treatment). n = 6/group, *P < 0.05 vs Veh, †P < 0.05 vs Drd5+/+. C Mitochondrial superoxide dismutase (SOD) protein (Mn-SOD and Cu/Zn-SOD) expression in isolated mitochondria from kidney cortices of Drd5−/− mice and their Drd5+/+ littermates. n = 4/group. There were no significant differences in SOD protein expression between Drd5+/+ and Drd5−/− mice