Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 17;52:109. doi: 10.1186/s13567-021-00978-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Ex vivo proliferation of SE-induced splenic CD4⁺ and CD8⁺ T cells.A The gating strategy shows the consecutive selection for viable cells (Live/Dead marker-negative), single cells (FSC-A vs FSC-H), lymphocytes (FSC-A vs SSC-A) and CD3⁺ T cells. T cells were subdivided into CD4⁺ and CD8⁺ T cells. The final gating step selects on T cell subsets which have divided at least once based on dilution of the cell proliferation dye CellTrace Violet (CTV). B The percentage of cells that have proliferated is shown for splenic CD4⁺ and C CD8⁺ T cells after 4 days of stimulation with different doses of formaldehyde-inactivated SE expressed in CFU/well, none stimulated controls (−), or after stimulation with anti-CD3, anti-CD28 and recombinant chicken IL-2 ( +). All splenocyte samples were stimulated and measured in triplicate for each of the conditions. Mean + SEM is shown; n = 2 for uninfected (uninf) and n = 3 for SE-infected (SE-inf) chickens.