Table 2.
Primers and TaqMan probe sequences used for RT-qPCR
| Cell type | Genes | NCBI Reference | TaqMan/SYBR-Green | Type | Sequence (5ʹ-3ʹ) |
|---|---|---|---|---|---|
| NK cells | NFIL3 | XM_017014743.1 | SYBR-Green | Forward | TGAATGCCATCAGTTGAGC |
| Reverse | GAGAGGCGGAGAATGTGAGT | ||||
| IL-7Rα | NM_001080106.1 | SYBR-Green | Forward | ATTCTGGGAAAGCAGGATCAAG | |
| Reverse | CTTACACAGTCGCTCCAGAGTTATTT | ||||
| PRF1 | XM_004945690.3 | SYBR-Green | Forward | ACCCGCACCAAAAGATGAAG | |
| Reverse | TAATTCGCACACCCCTAAACG | ||||
| GAPDH | NM_204305.1 | SYBR-Green | Forward | GTGGTGCTAAGCGTGTTATC | |
| Reverse | GCATGGACAGTGGTCATAAG | ||||
| APCs | CD14 | NM_001139478.1 | TaqMan | Forward | GGACGACTCCACCATTGACAT |
| Reverse | GGAGGACCTCAGGAACCAGAA | ||||
| Probe | AATGATCTTCCTGATTTGCAGACTGCCAA | ||||
| TLR4 | NM_001030693.1 | SYBR-Green | Forward | GTCCCTGCTGGCAGGAT | |
| Reverse | TGTCCTGTGCATCTGAAAGCT | ||||
| MERTK | NM_204988.1 | SYBR-Green | Forward | TGTGGAAGGATGGCAGGGAG | |
| Reverse | GCACGGATGCTGAATGTAGAGG | ||||
| MAFB | NM_001030852.1 | SYBR-Green | Forward | AGGACCGGTTCTCGGATGAC | |
| Reverse | CCTCGGAGGTGCCTGTTG | ||||
| INOS | NM_204961.1 | SYBR-Green | Forward | TGGGTGGAAGCCGAAATA | |
| Reverse | GTACCAGCCGTTGAAAGGAC | ||||
| ZBTB46 | XM_015296613.2 | SYBR-Green | Forward | CTGGACCTGTGGAAGAGGAAAC | |
| Reverse | CGGTAGTGGGAGGCAATCTC | ||||
| XCR1 | NM_001024644.2 | SYBR-Green | Forward | CCTTCGGGTGGATTTTTGGT | |
| Reverse | CGCTGTAGTAGCCAATGGAGAA | ||||
| FLT3 | NM_004119.3 | SYBR-Green | Forward | CATTCGGACCCAGTACATGTTTAC | |
| Reverse | TGAGCCGTAGAAGAGCAGGTATAA | ||||
| GAPDH | NM_204305.1 | SYBR-Green | Forward | GTGGTGCTAAGCGTGTTATC | |
| Reverse | GCATGGACAGTGGTCATAAG | ||||
| 28S | XR_00378040.1 | TaqMan | Forward | GGCGAAGCCAGAGGAAACT | |
| Reverse | GACGACCGATTTGCACGTC | ||||
| Probe | AGGACCGCTACGGACCTCCACCA |