Fig. 1.

LTCC-mediated increase in cytosolic [Ca²⁺] is slower in miR-221/222-transfected HL-1 cells. A Scheme of the applied superfusion protocol and typical response of a cell to 100 nM AngII, 25 mM KCl and 1 µM ionomycin (mimic control). Grey areas indicate areas used for calculation of area under the curve (AUC). B–D AngII elicits a transient increase in [Ca²⁺]_i. Peak height, percentage of non-responding cells and AUC are not significantly altered compared to mimic control. E–H KCl leads to a baseline shift of [Ca²⁺]_i up to a plateau level. The baseline shift is reduced by trend by miR-221/222. The area under the curve is significantly reduced in miR-221-transfected cells and miR-221/222 both lead to a significantly slower increase in [Ca²⁺]_i up to the plateau level. The time to max. is also shown in representative tracings (F, right side). The proportion of non-responding cells is significantly increased in miR-221-transfected cells. Straight line indicates median, dotted line indicates mean, *p  <  0.05, N  =  13–14 experiments per group (single data points), n  =  27–115 cells per experiment. mc mimic control