Fig. 3.

Calcium transient characteristics under control conditions are not affected by miR-221/222 in neoCM. A NeoCM form cell clusters with cardiac fibroblasts in culture. Immunofluorescence staining of ventricular neoCM after 6 days in culture. DAPI: nuclei, cardiac troponin T: cardiomyocytes, vimentin: fibroblasts. Scale bar indicates 50 µm. B Overview of most important monotonic transient analysis parameters calculated by IonWizard, sorted by the different phases of the transient. C–K Spontaneous or electrically evoked calcium transients were measured in monolayers under control conditions for 2 min. Monotonic transient analysis was performed to evaluate transient parameters for the baseline (C), departure (D, E), peak (F–I) and return phases (J, K) of the calcium transients. There is no significant difference between miR-221/222 and mimic control (mc). Straight line indicates median, dotted line indicates mean, mc: N  =  4–5, miR-221: N  =  6–7, miR-222: N  =  4