Fig. 1.

Body weight change and viral RNA load in serum and brain of young adult mice infected with ZIKV. TRIF^−/−xIPS-1^−/− mice were infected intravenously with 1 × 10⁵ PFUs of ZIKV strain PRVABC59 in 100 µL volume. Noninfected mice were used as controls. a Body weight changes of ZIKV-infected (○) and noninfected (■) mice. Results represent the mean ± SEM of 6 mice per group. b–c Subsets of mice were sacrificed on days 0 (noninfected), 3, 7, 10, and 14 post-infection and viral RNA load was determined in serum (b) and brain homogenates (c) by reverse transcriptase ddPCR. Results are the mean ± SEM of 5–6 mice per time point. The dotted lines represent the limit of detection of the reverse transcriptase ddPCR assay. ND, not detected. Statistical analyses were performed using a one-way analysis of variance with Tukey’s multiple comparison test. Results that are statistically different between indicated groups are shown as follows: * P < 0.05; *** P < 0.001