Table 3.
Morphological parameters of Iba1+/TMEM119+ microglia in both layers of the dorsal hippocampus CA1
| Parameters | Stratum radiatum | Stratum lacunosum-moleculare | ||||
|---|---|---|---|---|---|---|
| Day 0 Mean ± SEM |
Day 7 p.i. Mean ± SEM |
Day 10 p.i. Mean ± SEM |
Day 0 Mean ± SEM |
Day 7 p.i. Mean ± SEM |
Day 10 p.i. Mean ± SEM |
|
| Cell area (μm2) | 239.6 ± 19.0 | 250.3 ± 15.4 | 247.9 ± 12.9 | 146.8 ± 10.7 | 160.0 ± 93.5 | 150.2 ± 10.9 |
| Soma area (μm2) | 41.08 ± 2.55 | 48.18 ± 3.40 | 55.57 ± 3.89* | 42.30 ± 0.76 | 44.06 ± 2.47 | 51.12 ± 2.22* |
| Soma perimeter (μm) | 25.18 ± 0.92 | 27.74 ± 1.29 | 29.62 ± 1.15 (P = 0.0574) | 25.35 ± 0.26 | 26.16 ± 0.92 | 28.36 ± 0.68* |
| Arbor area (μm2) | 1576.2 ± 90.5 | 1443.2 ± 98.1 | 1931.5 ± 132.2# | 1204.2 ± 110.0 | 1046.9 ± 70.8 | 1351.6 ± 63.8# |
| Arbor perimeter (μm) | 186.0 ± 8.5 | 187.4 ± 11.1 | 227.0 ± 11.8 (P= 0.0505)# | 163.4 ± 12.2 | 158.0 ± 7.7 | 185.2 ± 4.6 |
| Morphological index (a.u.) | 0.028 ± 0.001 | 0.036 ± 0.001 | 0.031 ± 0.003 | 0.041 ± 0.003 | 0.046 ± 0.002 | 0.041 ± 0.002 |
| Circularity soma (a.u.) | 0.814 ± 0.009 | 0.794 ± 0.019 | 0.794 ± 0.010 | 0.830 ± 0.008 | 0.810 ± 0.012 | 0.800 ± 0.011 |
| Solidity soma (a.u.) | 0.904 ± 0.005 | 0.902 ± 0.006 | 0.899 ± 0.004 | 0.916 ± 0.003 | 0.914 ± 0.005 | 0.914 ± 0.005 |
| Aspect ratio soma (a.u.) | 1.653 ± 0.027 | 1.712 ± 0.053 | 1.638 ± 0.045 | 1.611 ± 0.049 | 1.725 ± 0.057 | 1.757 ± 0.054 |
| Roundness soma (a.u.) | 0.651 ± 0.013 | 0.626 ± 0.015 | 0.649 ± 0.015 | 0.666 ± 0.013 | 0.620 ± 0.017 | 0.621 ± 0.013 |
| Circularity arbor (a.u.) | 0.581 ± 0.015 | 0.538 ± 0.024 | 0.487 ± 0.035 | 0.572 ± 0.031 | 0.542 ± 0.017 | 0.509 ± 0.014 |
| Solidity arbor (a.u.) | 0.847 ± 0.007 | 0.827 ± 0.012 | 0.804 ± 0.018 | 0.847 ± 0.013 | 0.828 ± 0.007 | 0.810 ± 0.008* |
| Aspect ratio arbor (a.u.) | 1.498 ± 0.047 | 1.594 ± 0.031 | 1.478 ± 0.044 | 1.641 ± 0.047 | 1.757 ± 0.037 | 1.567 ± 0.051# |
| Roundness arbor (a.u.) | 0.693 ± 0.020 | 0.657 ± 0.010 | 0.706 ± 0.016 | 0.643 ± 0.017 | 0.612 ± 0.008 | 0.670 ± 0.021# |
a.u., arbitrary units; p.i., post-infection; SEM, standard error of the mean
Results are the mean ± SEM of 17–21 microglia per animal for a total of 5–6 mice per time point
Statistical analyses were performed using a one-way analysis of variance with Tukey’s multiple comparison test. * P < 0.05 day 10 compared with day 0 (noninfected mice); # P < 0.05 day 10 compared with day 7 p.i.. Both are indicated in bold