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. Author manuscript; available in PMC: 2021 Aug 17.
Published in final edited form as: Nat Immunol. 2011 Apr 10;12(5):399–407. doi: 10.1038/ni.2021

Figure 3.

Figure 3

Accelerated proliferation of Itch-mutant HSCs. (a) In vivo proliferative potential of wild-type and Itch−/− LSK subsets, as assessed by BrdU incorporation. Numbers above bracketed lines indicate percentages of proliferating (BrdU+) cells. (b) Ex vivo cell cycle status of wild-type and Itch−/− LSK cells, as assessed by pyronin Y and Hoechst staining. (c) In vitro proliferative potential of wild-type (shaded histograms) and Itch−/− (black lines) CD150+CD48− LSK cells, as assessed by CFSE dilution. (d) Ex vivo population expansion of wild-type and Itch−/− CD150+CD48− LSK cells in response to a cytokine ‘cocktail’. Data are representative of two (a,b) or five (c,d) independent experiments (mean ± s.e.m. of duplicates in d).