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Overexpression of EscV reduces bacterial fitness. Growth curves of WT EPEC (■), ΔescV (□), and EPEC ΔescV transformed with EscVwt-His (•), EscV-TMD5ex-His (△), EscV-TMD6ex-His (▲). Strains were grown at 37°C in DMEM (left panel) and LB (right panel) media with various IPTG concentrations (0, 0.1, and 0.25 mM). Optical density at 600 nm was measured every 30 min and plotted over time.
