TABLE 1.
Strains and plasmids used in this study.
| Strains | Description | References |
| WT EPEC | EPEC strain E2348/69, streptomycin resistant | Iguchi et al., 2009 |
| EPEC ΔescV | Non-polar deletion of escV | Gauthier et al., 2003 |
| EPEC ΔescN | Non-polar deletion of escN | Gauthier et al., 2003 |
| E. coli DH10B | For plasmid handling | Durfee et al., 2008 |
| E. coli FHK12 | E. coli strain in which the ctx promoter is fused to a lacZ gene | Kolmar et al., 1995 |
| E. coli PD28 | A malE-deficient E. coli strain | Duplay and Szmelcman, 1987 |
| E. coli BL21 (λDE3) | For protein expression | Promega |
| Plasmids | ||
| pEscVwt (pACYC184) | Untagged EscV in pACYC184 | Gauthier et al., 2003 |
| pEscVwt-V5 (pACYC184) | V5 C-terminal tagged EscV in pACYC184 | This study |
| pEscVwt-HSV (pACYC184) | HSV C-terminal tagged EscV in pACYC184 | Gauthier et al., 2003 |
| pEscVwt-V5 (pSA10) | V5 C-terminal tagged EscV in pSA10 | This study |
| pEscVwt-HA (pSA10) | HA C-terminal tagged EscV in pSA10 | This study |
| pEscVwt-His (pSA10) | His C-terminal tagged EscV in pSA10 | This study |
| pEscV-TMD5ex-His (pSA10) | C-terminal tagged EscV with an 7L9A sequence instead of the original TMD5 in pSA10 | This study |
| pEscV-TMD6ex-His (pSA10) | C-terminal tagged EscV with an 7L9A sequence instead of the original TMD6 in pSA10 | This study |
| pEscV-TMD5ex-V5 (pSA10) | C-terminal tagged EscV with an 7L9A sequence instead of the original TMD5 in pSA10 | This study |
| pEscV-TMD6ex-V5 (pSA10) | C-terminal tagged EscV with an 7L9A sequence instead of the original TMD6 in pSA10 | This study |
| pEscVG213A-V5 (pSA10) | V5 C-terminal tagged EscV with a point mutation in position 213 in pSA10 | This study |
| pEscVG217A-V5 (pSA10) | V5 C-terminal tagged EscV with a point mutation in position 217 in pSA10 | This study |
| pEscVG213L-V5 (pSA10) | V5 C-terminal tagged EscV with a point mutation in position 213 in pSA10 | This study |
| pEscVG217L-V5 (pSA10) | V5 C-terminal tagged EscV with a point mutation in position 217 in pSA10 | This study |
| pToxR-GpA-MBP | The GpA TMD sequence inserted between ToxR and MBP | Langosch et al., 1996 |
| pToxR-Tar1-MBP | Tar TMD1 sequence inserted between ToxR and MBP | Sal-Man et al., 2004 |
| pToxR-A16-MBP | A sequence of 16 alanine residues inserted between ToxR and MBP | Langosch et al., 1996 |
| pToxR-TMD1-MBP | TMD1 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD2-MBP | TMD2 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD3-MBP | TMD3 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD4-MBP | TMD4 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD5-MBP | TMD5 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD6-MBP | TMD6 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD7.1-MBP | TMD7.1 of EscV inserted between ToxR and MBP | This study |
| pToxR-TMD7.2-MBP | TMD7.2 of EscV inserted between ToxR and MBP | This study |