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. 2021 Aug 17;19:353. doi: 10.1186/s12967-021-03018-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — FGF11 regulates HIF-1α expression for NSCLC progression. A Gene Set Enrichment Analysis (GSEA) of the hypoxia signaling pathway genes in FGF11 positive and negative samples usingGSE81089 RNA-seq data. B qPCR analysis of HIF-1α mRNA expression after FGF11 knockdown in A549 and NCI-H460 cells. C Western blot analysis of HIF-1α protein level after FGF11 knockdown in A549 and NCI-H460 cells. D qPCR analysis of HIF-1α mRNA expression in A549 and NCI-H460 cells with FGF11 overexpression. E Western blot analysis of HIF-1α protein level in A549 and NCI-H460 cells with FGF11 overexpression. F qPCR quantification of HIF-1α mRNA level after siRNA transfection. G CCK-8 proliferation assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. H Colony formation assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. I wound healing assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. J Transwell migration assay in A549 and NCI-H460 cells transfected with FGF11 and/or HIF-1α siRNA. The results are represented as mean ± standard derivation. Data are summary of 3 independent experiments. *P < 0.05, ** P < 0.01, ***P < 0.005 vs. sh-NC or empty vector-transfected cells