Table 1.
Distribution of TYRP1 in normal and HPS-5 melanocyte organelles, using immunoEM
| Intracellular compartment | Particles detected by MEL5 labeling (%)1 | Particles detected by αPEP1 labeling (%)2 | ||
|---|---|---|---|---|
| Normal | HPS-5 | Normal | HPS-5 | |
| Melanosomes | 52 (30) | 1 (3) | 42 (36) | 4 (8) |
| Small vesicles not in the Golgi region | 30 (17) | 26 (68) | 19 (16) | 22 (44) |
| Golgi stacks and associated vesicles | 14 (8) | 0 (0) | 23 (19) | 5 (10) |
| Early endosomes | 21 (12) | 2 (5) | 15 (13) | 3 (6) |
| Late endosomes, MVBs, lysosomes | 45 (26) | 5 (13) | 13 (11) | 11 (22) |
| Small tubules | 12 (7) | 4 (11) | 6 (5) | 5 (10) |
| Total | 174 (100) | 38 (100) | 118 (100) | 50 (100) |
HPS-5, Hermansky-Pudlak syndrome 5; MVBs, multivesicular bodies.
1
A total of six normal and four HPS-5 cells labeled with MEL5 were scored. Percentage of total is indicated in parenthesis.
2
Five normal and six HPS-5 cells labeled with αPEP1 were scored. Percentage of total is indicated in parenthesis.