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. Author manuscript; available in PMC: 2021 Aug 17.
Published in final edited form as: Nat Cell Biol. 2013 Dec 8;16(1):55–65. doi: 10.1038/ncb2883

Figure 7.

Figure 7

Calcium channels are involved in necroptosis. (a) HT29 cells were treated with nifedipine (100 μm), NiCl2 (1 mM), LaCl3 (100 μm) or 2-APB (50 μm) and TSZ for 24 h. Cell survival was determined by flow cytometry of PI staining. (b,c) JurkatFADD−/− cells (b) or U937 cells (c) were pre-treated with LaCl3(100 μM), NAC (5 mM) or LaCl3 plus NAC. Then, the cells were treated with TSZ as indicated. After 10 h, cell survival was determined by PI staining. (d) JurkatFADD−/− cells were pre-treated with the increased concentration of LaCl3 (200 μM) and then treated with TSZ as indicated. After 10 h, cell survival was determined by PI staining. Results shown are averages ± s.e.m. from three independent experiments. *P < 0.05, **P < 0.01. Statistics source data for this figure can be found in Supplementary Table 1.