FIGURE 4.

Plasma membrane localization of OsHAK8. The GFP coding sequence was fused to the C-terminus of the OsHAK8 coding region in the pCambia2300 vector and then transformed into rice mesophyll protoplasts. GFP alone in the same vector was used as a control. Signals from GFP, OsHAK8-GFP, and SP1-RFP (a plasma membrane localization marker) were imaged using a Zeiss LSM880 confocal laser scanning microscope. Columns 1–4 show GFP signals, SP1-RFP signals, merged images of GFP and RFP signals, and bright-field differential interference contrast (DIC), respectively. For each localization experiment, ≥ 30 individual cells were analyzed using a Zeiss LSM880 confocal laser scanning microscope (Carl Zeiss). Bar = 10 μm.