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. 2021 Aug 3;12:706934. doi: 10.3389/fmicb.2021.706934

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Copyright © 2021 Böhme, Heroven, Lobedann, Guo, Stolle and Dersch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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H-NS has a positive influence on csrC. (A) Y. pseudotuberculosis strain YPIII and YP50 (ΔymoA) harboring the empty vector (V), a hns* (pAKH31) or hns⁺ (pAKH74) overexpression construct were cultivated overnight in LB medium at 25°C. Total RNA of the strains was prepared, separated on agarose gels and a specific probe was used to detect the CsrC RNA by Northern blotting. The 16S rRNA is shown as RNA loading control. (B) Expression of csrC(+4)-lacZ and csrC(+81)-lacZ was analyzed in overnight cultures of Y. pseudotuberculosis strains YPIII (wild-type) and YP50 (ΔymoA) harboring vector pACYC184, pAKH85 or its hns⁺ derivative pAKH74 grown in LB at 25°C. β-galactosidase activity from the overnight cultures was determined and is given as relative expression for comparison. The data represent the average ± SD from at least three different experiments each done in duplicate. Data were analyzed by the ANOVA test, ^∗∗∗P < 0.001.