FIGURE 8.

Comparison of the environmental control of CsrA, YmoA, and CsrC synthesis. Y. pseudotuberculosis strain YPIII was diluted 1:100 from an overnight culture and was grown in LB at 25 and 37°C. Every 2 h, samples were withdrawn for whole cell extract and total RNA preparations (A) and the OD₆₀₀ of the cultures was determined to monitor growth (B). (A) Total RNA of the samples was separated on agarose gels and the CsrC RNA was detected by Northern blotting using a CsrC-specific probe. Whole cell extracts were separated on 18% TRICINE polyacrylamide gels and analyzed by Western blotting with a polyclonal antibody directed against YmoA and CsrA. The CsrC RNA band and the YmoA and CsrA protein bands are indicated by arrows. (B) OD₆₀₀ of the cultures used for the upper analysis was plotted over time to illustrate and compare the different growth phases.