FIGURE 8.
Comparison of the environmental control of CsrA, YmoA, and CsrC synthesis. Y. pseudotuberculosis strain YPIII was diluted 1:100 from an overnight culture and was grown in LB at 25 and 37°C. Every 2 h, samples were withdrawn for whole cell extract and total RNA preparations (A) and the OD600 of the cultures was determined to monitor growth (B). (A) Total RNA of the samples was separated on agarose gels and the CsrC RNA was detected by Northern blotting using a CsrC-specific probe. Whole cell extracts were separated on 18% TRICINE polyacrylamide gels and analyzed by Western blotting with a polyclonal antibody directed against YmoA and CsrA. The CsrC RNA band and the YmoA and CsrA protein bands are indicated by arrows. (B) OD600 of the cultures used for the upper analysis was plotted over time to illustrate and compare the different growth phases.