KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Raptor | Cell Signaling Technology | Cat# 2280; RRID: AB_561245 |
| ACC | Cell Signaling Technology | Cat# 3676; RRID: AB_2219397 |
| ACLY | Cell Signaling Technology | Cat# 4332; RRID: AB_2223744 |
| FASN | Cell Signaling Technology | Cat# 3180; RRID: AB_2100796 |
| α-Tubulin | Cell Signaling Technology | Cat# 2125; RRID: AB_2619646 |
| PPARγ | Santa Cruz biotechnology | Cat# sc-7273; RRID: AB_628115 |
| UCP1 | Abcam | Cat# ab10983; RRID: AB_2241462 |
| Chemicals, peptides, and recombinant proteins | ||
| D-GLUCOSE (U-13C6, 99%) | Cambridge Isotope Laboratories | Cat#CLM-1396-PK |
| [1,2-3H]-deoxy-D-glucose | Perkin Elmer | NET328A001MC |
| Critical commercial assays | ||
| Glucose meter | Bionime | GE100 |
| Ultrasensitive insulin ELISA kit | Crystal Chem | 90060 |
| Deposited data | ||
| Data S1. Isotope tracing and Metabolomics upon temperature acclimation, related to Figures 1, 2, 3, 4, 5, 6, and S1-S9 | This Study | Supplemental information |
| Data S2. Isotope tracing and Metabolomics upon acute cold, related to Figures 7 and S10 | This Study | Supplemental information |
| RNA sequencing raw data upon temperature acclimation, related to Figures 2, 4, S3-S5, S7, and S8 | Sanchez-Gurmaches et al., 2018 (PMID: 29153407) | NCBI Gene Expression Omnibus: GSE96681 |
| Experimental models: Organisms/Strains | ||
| Mouse: C57BL/6 | Jackson labs | 000664 |
| Software and algorithms | ||
| Graphpad | GraphPad Software | Graphpad.com |
| Adobe Illustrator | Adobe | Adobe.com |
| MAVEN software | http://genomics-pubs.princeton.edu/mzroll/index.php | N/A |
| MetaboAnalyst 3.0, 4.0, 5.0 | https://www.metaboanalyst.ca | N/A |
| Other | ||
| Rodent incubators | Power Scientific | RIT33SD |
KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Raptor | Cell Signaling Technology | Cat# 2280; RRID: AB_561245 |
| ACC | Cell Signaling Technology | Cat# 3676; RRID: AB_2219397 |
| ACLY | Cell Signaling Technology | Cat# 4332; RRID: AB_2223744 |
| FASN | Cell Signaling Technology | Cat# 3180; RRID: AB_2100796 |
| α-Tubulin | Cell Signaling Technology | Cat# 2125; RRID: AB_2619646 |
| PPARγ | Santa Cruz biotechnology | Cat# sc-7273; RRID: AB_628115 |
| UCP1 | Abcam | Cat# ab10983; RRID: AB_2241462 |
| Chemicals, peptides, and recombinant proteins | ||
| D-GLUCOSE (U-13C6, 99%) | Cambridge Isotope Laboratories | Cat#CLM-1396-PK |
| [1,2-3H]-deoxy-D-glucose | Perkin Elmer | NET328A001MC |
| Critical commercial assays | ||
| Glucose meter | Bionime | GE100 |
| Ultrasensitive insulin ELISA kit | Crystal Chem | 90060 |
| Deposited data | ||
| Data S1. Isotope tracing and Metabolomics upon temperature acclimation, related to Figures 1, 2, 3, 4, 5, 6, and S1-S9 | This Study | Supplemental information |
| Data S2. Isotope tracing and Metabolomics upon acute cold, related to Figures 7 and S10 | This Study | Supplemental information |
| RNA sequencing raw data upon temperature acclimation, related to Figures 2, 4, S3-S5, S7, and S8 | Sanchez-Gurmaches et al., 2018 (PMID: 29153407) | NCBI Gene Expression Omnibus: GSE96681 |
| Experimental models: Organisms/Strains | ||
| Mouse: C57BL/6 | Jackson labs | 000664 |
| Software and algorithms | ||
| Graphpad | GraphPad Software | Graphpad.com |
| Adobe Illustrator | Adobe | Adobe.com |
| MAVEN software | http://genomics-pubs.princeton.edu/mzroll/index.php | N/A |
| MetaboAnalyst 3.0, 4.0, 5.0 | https://www.metaboanalyst.ca | N/A |
| Other | ||
| Rodent incubators | Power Scientific | RIT33SD |