Figure 4.

(a) Total leukocyte count was not significantly different in Ly6G group from control at any time point, despite being relatively lower at 24 h. Differential neutrophil count was significantly different between two groups only at the 24-h time point. In control animals, there was a trend of increasing neutrophil counts after ischemia over one day although statistically nonsignificant. Boxes show medians and interquartile range, whiskers show 5–95th percentiles. (b) Penumbral blood flow changes during MCAO and reperfusion in control and Ly6G groups, measured by prD-OCT and expressed as percentage of baseline flow. The decrease in blood flow was almost the same in both treatment groups, but blood flow recovered to a significantly higher level at 2 and 24 h after reperfusion in the Ly6G-treated group (c) Total number of capillary segments in experimental groups in ischemic penumbra, measured at baseline and at different experimental time points. Although the anti-Ly6G group seemed to have a relatively higher number of capillary segments after reperfusion, this difference was not significant. (d–f) Longitudinal monitoring of various stall parameters at different time points during occlusion and reperfusion in ischemic penumbra. Baseline and ischemia conditions were almost identical in control and Ly6G groups, but with an improvement starting (but marginally significant) as early as 1 h, which became significant at 2 h and 24 h after reperfusion. (g–h) MCA pial branch flow velocities and capillary flow velocities were significantly higher in anti-Ly6G-treated animals at 2 h and 24 h after reperfusion. (i) Pial arterial diameters measured from 4th order MCA branches (normalized to baseline diameters) did not show significant changes between treatment groups. Animal counts: For the 24-h timepoint, n = 6 for both treatment groups. For the other time points, n = 13 and n = 11 for control and anti-Ly6G, respectively. Significance: (**) (##) p < 0.01, (*)(#) 0.01≤p < 0.05, (ƒ)(§) 0.05≤p < 0.09, (n.s.) p ≥ 0.09.