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. 2021 Aug 3;9:679483. doi: 10.3389/fbioe.2021.679483

FIGURE 5.

FIGURE 5

Vector distribution and miRNA expression in mice brain after IV or intrastriatal (IStr) delivery. (A) A graphical representation of vectors used in this study. The expression cassette used consisted of CAG-promoter-miRNA and poly(A) tail. (B) Administration routes at which mice were either administered intravenously (IV) or IStr. (C) Distribution in the striatum. A significantly higher amount of vector DNA was observed in the striatum and cortex of mice that received AAV5 IStr. For AAV5, direct administration results in significantly higher distribution, while for AAV9-PhP.B, similar amounts of the vector were retrieved on average. (D) Distribution in the cortex. In the cortex, the same pattern is observed as in the striatum. The IStr administration of AAV5 results in higher amounts of vector DNA than the IV administration of AAV9-PhP.B or AAV5 IV. (E) miRNA levels in the striatum and (F) miRNA levels in cortex. The vector DNA results translate to miRNA expression levels. In this study, we also observed higher miRNA levels in mice administered AAV5 IStr compared with AAV9-PhP.B IV and AAV5-PhP.B. ND stands for not detected. Statistical analysis was performed by the one-way ANOVA, followed by the Bonferroni multiple comparisons test. Asterisks indicate a statistical significance of ∗p ≤ 0.05, ∗∗p < 0.005, ∗∗∗p ≤ 0.001, and ****p < 0.0001.