KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | |||
|---|---|---|---|---|---|
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Antibodies
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| Mpp11 | Cell Signaling | Cat#12844, RRID: AB_2798042 | |||
| Hsp70L1 | Abcam | Cat#ab108612, RRID: AB_10866191 | |||
| GAPDH | Cell Signaling | Cat#2118S, RRID: AB_561053 | |||
| PERK | Cell Signaling | Cat#3192, RRID: AB_2095847 | |||
| Calnexin | Cell Signaling | Cat#2679; RRID: AB_2228381 | |||
| BiP | Cell Signaling | Cat#3177, RRID: AB_2119845 | |||
| Rpl10 | Abnova | Cat#PAB17331, RRID: AB_10643619 | |||
| SRP54 | BD | Cat#610940, RRID:AB_398253 | |||
| IRE1α | Cell Signaling | Cat#3294, RRID: AB_823545 | |||
| Hsp70 | Cell Signaling | Cat#4872, RRID: AB_2279841 | |||
| Pelo | Proteintech | Cat#10582-1-AP, RRID: AB_2236833 | |||
| Flag | Sigma | Cat#F1804, RRID: AB_262044 | |||
| IRDye 680RD secondary antibodies goat anti-mouse IgG |
Li-Cor | Cat#926-68070, RRID: AB_10956588 | |||
| IRDye 680RD secondary antibodies goat anti-rabbit IgG |
Li-Cor | Cat#926-68071, RRID: AB_10956166 | |||
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Chemicals, peptides, and recombinant proteins
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| Thapsigargin | Sigma | Cat#T9033 | |||
| Celastrol | Sigma | Cat#C0869 | |||
| Cycloheximide | Sigma | Cat#C7698 | |||
| Homoharringtonine | Sigma | Cat#SML1091-10MG | |||
| Doxycycline | Sigma | Cat#D9891 | |||
| Digitonin | Millipore | Cat#300410 | |||
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Critical commercial assays
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| CellTiter 96 AQueous one solution cell proliferation assay (MTS) | Promega | Cat#G3582 | |||
| High-capacity cDNA reverse transcription kit | ThermoFisher Scientific | Cat#4368814 | |||
| Click-iT protein reaction buffer kit | ThermoFisher Scientific | Cat#C10276 | |||
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Deposited data
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| Ribosome profiling | This paper | GEO: GSE173852 | |||
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Experimental models: Cell lines
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| HeLa Tet-on | Clontech | N/A | |||
| HEK293 | ATCC | CRL-1573 | |||
| HEK293T | ATCC | CRL-11268 | |||
| T-REx293 IRE1-GFP | Li et al., 2010 | N/A | |||
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Oligonucleotides
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| siRNA Hsp70L1 target sequence-1: CAGAAAUACAUCGCGGAAA |
Dharmacon | M-021084-01-0005 | |||
| siRNA Hsp70L1 target sequence-2: UAACAUCGGUGGUGCACAU |
Dharmacon | M-021084-01-0005 | |||
| siRNA Hsp70L1 target sequence-3: GGAAAUGCGCGAGCCAUGA |
Dharmacon | M-021084-01-0005 | |||
| siRNA Hsp70L1 target sequence-4: GUAUUGGGCUCAGAUGCAA |
Dharmacon | M-021084-01-0005 | |||
| siRNA Mpp11 target sequence-1: GAACCAAGAUCAUUAUGCA |
Dharmacon | M-025435-02-0005 | |||
| siRNA Mpp11 target sequence-2: GAAAUCAACUGGUGGAGGU |
Dharmacon | M-025435-02-0005 | |||
| siRNA Mpp11 target sequence-3: GAACUUGUCGAGAUGGUAA |
Dharmacon | M-025435-02-0005 | |||
| siRNA Mpp11 target sequence-4: AGGACUGCAUGAAACGAUA |
Dharmacon | M-025435-02-0005 | |||
| ON-TARGETplus Non-targeting Control siRNAs #1: UGGUUUACAUGUCGACUAA |
Horizon | D-001810-01-20 | |||
| Additional primer sequences | Tables S1 and S2 | ||||
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Recombinant DNA
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| Plasmid: pcDNA3.1(+)-Flag-uXbp1-A | GenScript | N/A | |||
| Plasmid: pcDNA3.1(+)-Flag-sXbp1-A | GenScript | N/A | |||
| Plasmid: pJI204-T7-GFP-A | Yang et al., 2019 | N/A | |||
| Plasmid: pJI204-T7-Luc-A | Yang et al., 2019 | N/A | |||
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Software and algorithms
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| Fiji/ImageJ | NIH image | https://imagej.net/Fiji | |||
| Prism 8 | GraphPad | https://www.graphpad.com/scientificsoftware/prism/ | |||