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. Author manuscript; available in PMC: 2023 Oct 3.
Published in final edited form as: J Am Chem Soc. 2021 Aug 10;143(33):13205–13211. doi: 10.1021/jacs.1c05435

Figure 5. PRE and NOE evidence of FP insertion into bicelles.

Figure 5.

About one nitroxide spin-labeled 14-doxyl PC probe per leaflet was added to the bicelles. Proximity to the nitroxide label deep in the bicelles is symbolized with red for strong paramagnetic broadening of the amide NMR line. Gray symbolizes minimal broadening. (A) The PREs are estimated from amide NMR peak heights before and after addition of 14-doxyl PC. The 15N HSQC used for detection was modified to include a 1H PROJECT CPMG train of 8 ms 39,52. (B) Residues with PREs to amide groups ≥ 0.7 in A are red while those between 0.7 and 0.3 are pink. Side chains that appear to have NOEs to phospholipid acyl chains (Fig. S10) are plotted. (C) A hypothesis regarding insertion of FP in a leaflet of DMPC is shown with lipids symbolized by dots. A faint mesh marks the surface of FP. The CHARMM-GUI Membrane Builder53 was used.