Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 9;1(1):15–37. doi: 10.1021/acsnanoscienceau.1c00009

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Schematic representation of a BRET biosensor. The donor and acceptor have overlapping emission and absorption spectra. Nonradiative energy is only transferred between a light-emitting donor and a fluorescent acceptor when the two are less than 10 nm apart. Proteins X and Y are interacting proteins fused to the donor and acceptor and allow for the two substrates to interact and emit light or BRET signal from the acceptor. Protein interactions can be determined by BRET by comparing donor and acceptor emission wavelengths, which differ in light intensity over wavelength. Adapted with permission from ref (60). Copyright 2008 Wiley-VCH Verlag GmbH & Co.