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. 2021 Aug 9;1(1):15–37. doi: 10.1021/acsnanoscienceau.1c00009

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© 2021 The Authors. Published by American Chemical Society

Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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Schematic representation of different BRET methods. Each method is characterized by different BRET donors and acceptors, spectral properties, and applications. BRET2 uses coelenterazine 400a, which shifts the emission of RLuc to that of GFP and has a larger separation of donor and acceptor emission peaks. Enhanced BRET2 has a light intensity stronger than that of BRET2. BRET3 uses FLuc and DsRed, which has a prolonged emission signal. QD-BRET uses quantum dots and has a distinct separation of donor and acceptor emission peaks. GFP2: green fluorescent protein 2. RLuc8: variant of Renilla luciferase. DsRed: red fluorescent protein. 6-His: polyhistidine tag. QD or QDot: quantum dots. Adapted with permission from ref (60). Copyright 2008 Wiley-VCH Verlag GmbH & Co.