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. 2021 Aug 9;1(1):15–37. doi: 10.1021/acsnanoscienceau.1c00009

Figure 16.

Figure 16

Schematic representation of wild-type MAVS and MAVS with cysteine-508 residue mutated to arginine in a BRET assay. NS3/4A cleaves MAVS at cysteine-508 and prevents MAVS–MAVS interactions. The wild-type MAVS can be cleaved, and the mutated RLuc-MAVS and Venus-MAVS cannot be cleaved. The BRET assay was set up to ultimately detect MAVS homo-oligomerization and used RLuc as the donor and Venus as the acceptor, both of which are fused to MAVS. MAVS: mitochondrial antiviral signaling. NS3/4A: hepatitis C virus protease. PRR: proline-rich region. CARD: caspase activation and recruitment domain. OM: mitochondrial outer membrane. Venus: yellow fluorescent protein. Adapted with permission from ref (86). Copyright 2013 Elsevier B.V.