Figure 18.

Schematic representation of an example assay with circularly permuted (CP) luciferase at Asp234 (CP₂₃₄-Luc). CP-luciferase was created by joining the N- and C-termini of luciferase via inserting a protease recognition sequence. (A) New N- and C-termini were added, respectively, at amino acid positions 234 and 233. (B) Cyclization of the luciferase lowers its luciferase activity. Cognate protease digestion at the protease recognition sequence reactivates the CP₂₃₄-Luc protein. Adapted with permission from ref (90). Copyright 2008 Wigdal et al.; Licensee Bentham Open.