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Construction of a split luciferase PCA. In this example, the N-terminus (ELucN) and C-terminus (ELucC) of enhanced green-emitting luciferase (ELuc) are fused to proteins of interest, FK506-binding protein (FKBP) and FKBP rapamycin binding (FRB) domain, respectively. These two proteins interact in the presence of rapamycin, which allows for the ELuc fragments to be brought into close proximity and restored bioluminescence.
