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. 2021 Aug 9;1(1):15–37. doi: 10.1021/acsnanoscienceau.1c00009

Figure 21.

Figure 21

Schematic representation of the generation of 233H, 358H, 233DH, and 358DH biosensors for detecting HAV 3C cysteine protease activity. 233-HAV has the recognition sequence for HAV 3Cpro (red rectangle) connecting the two halves of FLuc at amino acid positions 4 and 544, while 233-DnaE-HAV has additional DnaE fragments (black rectangles) attached to the N- and C-termini of FLuc. All four plasmids are cleaved and give a signal when they are cotransfected with HAV 3C protease plasmid. 233DH and 358DH only have complementation when both DnaE and HAV 3Cpro are present. H: hepatitis A virus. D: fusion to DnaE. HAV: hepatitis A virus. 3Cpro: HAV 3C cysteine protease. Npu: Nostoc punctiforme. DnaE: DNA polymerase III intein. Adapted with permission from ref (91). Copyright 2017 and Elsevier Inc.