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Illustration of the NLuc system for split luciferase assays. The NLuc enzyme can be divided into two subunits: a 1.3 kDa peptide (red) and a larger 18 kDa polypeptide called LgBiT (blue). Mutations to the small 1.3 kDa peptide yielded two well-known fragments: SmBiT and HiBiT (not shown). Adapted from ref (30). Copyright 2016 American Chemical Society.
