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. 2021 Aug 9;1(1):15–37. doi: 10.1021/acsnanoscienceau.1c00009

Figure 9.

Figure 9

Schematic representation of split luciferase assay to measure cell–cell fusion mediated by the HSV glycoprotein. Effector cells are transfected with the HSV glycoprotein and one-half of RLuc and GFP. Target cells are transfected with the other half of RLuc and GFP. After 24 h, RLuc substrate coelenterazine (EnduRen) is added and the two cell types are mixed. If the cells fuse together, the RLuc halves can reunite to produce a bioluminescence signal. B78: mouse melanoma cells. C10: human colon cells. DSP: dual split protein. RLuc8: variant of RLuc. B, D, HL (on B78): gB, gD, and a heterodimer gH/gL which are four glycoproteins contained within the viral envelope. R (on C10): receptor.