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. 2021 Aug 9;1(1):15–37. doi: 10.1021/acsnanoscienceau.1c00009

Table 2. Applications of Luciferase-Based Biosensors and Their Assay Format.

application of biosensor type of biosensor assay format type of Luciferase ref
detection of protein dimerization split luciferase in vitro Renilla luciferase (23)
  split luciferase in vitro Gaussia luciferase (44)
  BRET in vitro Renilla luciferase (83,86)
analysis of viral entry split luciferase in vitro nanoluciferase (34,122,123)
  split luciferase in vitro Renilla luciferase (48,49,54,55,57)
creation of reporter viruses for screening of antiviral agents split luciferase in vitro/in vivo nanoluciferase (35)
screening for inhibitors of viral protease split luciferase in vitro firefly luciferase (40)
  circularly permutated luciferase in vitro Renilla luciferase (95)
  cyclic luciferase in vitro firefly luciferase (91)
  BRET in vitro Renilla luciferase (71,73)
  split luciferase in vivo/in vitro firefly luciferase (28)
screening for inhibitors of viral protein kinase split luciferase in vitro Renilla luciferase (47)
screening for inhibitors of viral polymerase split luciferase in vitro firefly luciferase (18)
studying immune system downregulation by viral infection BRET in vitro Renilla luciferase (72)
quantifying inhibition of interferon signaling dual luciferase in vitro firefly luciferase/Renilla luciferase (103)
studying interaction between virus and host proteins split luciferase in vitro Gaussia luciferase (42,59)
  split luciferase in vitro firefly luciferase (29)
  split luciferase in vitro nanoluciferase (124)
studying effects of sequence changes of genes split luciferase in vitro Gaussia luciferase (44,59)
  dual luciferase in vitro firefly luciferase/Renilla luciferase (100)
studying viral protein structure BRET in vitro Renilla luciferase (84)
identifying microRNA binding sites dual luciferase in vitro firefly luciferase/Renilla luciferase (109)
screening for inhibitors for protein dimerization split luciferase in vitro Renilla luciferase (23)
detecting viral protease activity split luciferase in vitro/in vivo firefly luciferase (28)
detection of antibodies against SARS-CoV-2 split luciferase in vitro nanoluciferase (127)
  split luciferase in vitro nanoluciferase (128)
characterizing immune response against viral infection dual luciferase in vitro firefly/Renilla luciferase (108)
detection of antibody seroconversion split luciferase in vitro nanoluciferase (125)
  split luciferase in vitro nanoluciferase (126)