Hershberger assay: Dosing effect of compounds 16 (30 mg/kg),
19 (5 mg/kg), (S)-26
(30 mg/kg), and (R)-28 (30 mg/kg)
compared with control group 1 (enzalutamide, 30 mg/kg)
and (R)-29 (5 mg/kg) and
(S)-27 (5 mg/kg) compared with
control group flutamide (3 mg/kg) on ASOs. The ASO development of
seminal vesicles and coagulating glands (SVCG) and the ventral
prostate (VP) is shown. The compound-dependent suppression of ASOs is
significant for each compound tested (p ≤
0.0001, t-test/Mann–Whitney). Data are the
mean ± SD (n = 6).